Не должна:
http://support.microsoft.com/default.aspx?scid=kb;en-us;283037

The preceding information is valid for programs that run when the /3GB switch is used. A program that requests 3 GB of memory is more likely to be able to have more of its memory remain in physical memory rather than be paged out. This increases the performance of programs that are capable of using the /3GB switch. The exception is when the /3GB switch is used in conjunction with the /PAE switch. In this case, the operating system does not use any memory in excess of 16 GB. This behavior is caused by kernel virtual memory space considerations. Thus, if the system restarts with the /3GB entry in the Boot.ini file, and the system has more than 16 GB of physical memory, the additional physical random access memory (RAM) is not used by the operating system. Restarting the computer without the /3GB switch enables the use of all the physical memory.