<asp:Datagrid id="Datagrid1" runat="server"> 
 <Columns> 
 <asp:TemplateColumn> 
 <ItemTemplate> 
 <asp:Label id="Label1" Runat ="server" > <%#getCount%> </asp:Label> 
 </ItemTemplate> 
 </asp:TemplateColumn> 
 </Columns> 
 </asp:Datagrid> 

int count = 0 ; 
protected int getCount() 
{ 
     count = count +  1 ; 
     return count; 
} 
private void Page_Load(object sender, System.EventArgs e) 
{ 
//Populate the Datagrid populating data from the database 
//.... 
}