HCERTCHAINENGINE         hChainEngine;

CERT_CHAIN_ENGINE_CONFIG chainConfig;

chainConfig.cbSize                    = sizeof(CERT_CHAIN_ENGINE_CONFIG);
chainConfig.hRestrictedRoot           = NULL;
chainConfig.hRestrictedTrust          = hStore; //Хранилище сертификатов в памяти (из файла подписи)
chainConfig.hRestrictedOther          = NULL;
chainConfig.cAdditionalStore          = 0;
chainConfig.rghAdditionalStore        = NULL;
chainConfig.dwFlags                   = 0;
chainConfig.dwUrlRetrievalTimeout     = 0;
chainConfig.MaximumCachedCertificates = 0;
chainConfig.CycleDetectionModulus     = 0;

PCCERT_CHAIN_CONTEXT     pChainContext;

if (!CertCreateCertificateChainEngine(&chainConfig, &hChainEngine))
{
   ...
}